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This experiment shows that the Rho/ROCK pathway is involved in regulation of VEGFR-2 endocytosis and subsequent activation in response to matrix stiffness

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To elucidate the role of R-loops in DNA replication stress induced by overexpression of HRASV12 or cyclin E1, we introduced the retroviruses encoding these oncogenes into U2OS T-REx cells harboring an RNASEH1 transgene under the control of a doxycycline-regulated CMV promoter (U2OS T-REx [RNH1-GFP]) 32