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Aflatoxin B1 [121], for instance, induces a characteristic G → T transversion in codon 249 of TP53, a hallmark of hepatocellular carcinoma [122]
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For this interaction to occur, the structural requirement in the enzyme is the presence of a nucleophilic amino acid side-chain close to the glutathione-docking motif and in a crucial position to interact with the electrophilic motif of glutathione acrylate, as shown in Scheme 9
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